faustovirus capping enzyme (New England Biolabs)

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New England Biolabs faustovirus capping enzyme
Faustovirus Capping Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/faustovirus+capping+enzyme/Faustovirus+Capping+Enzyme/pmc13107127-189-7-11
Average 95 stars, based on 29 article reviews

faustovirus capping enzyme - by Bioz Stars, 2026-09

95/100 stars

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Synthesized:

Article Title: Single intramuscular injection of self-amplifying RNA of Nppa to treat myocardial infarction
Article Snippet: 5-Methyl-CTP (B7967, ApexBio Technology) and pseudouridine-UTP (B7972, ApexBio Technology) were added to the IVT reaction mixture and the final molar ratio of modified nucleotides to standard nucleotides was 1:2. .. Subsequently, the synthesized RNAs were capped with Faustovirus Capping Enzyme ( M20081 , NEB) and tailed with E. coli Poly(A) Polymerase (M0276, NEB). .. After each step, the reaction products were purified using Monarch RNA Cleanup Kit (T2050, NEB).

Purification:

Article Title: A Universal, AI-based Design Framework for Efficient Manufacturing of mRNA Therapeutics
Article Snippet: To test RNA translatability, batch IVT was performed with 100% substitution of m1y (Trilink N-1081). .. Subsequently, these purified RNA were capped and polyadenylated using Faustovirus Capping Enzyme (NEB) and E. coli Poly(A) Polymerase (NEB) respectively according to manufacturer’s protocols. .. Human 293T cells were maintained in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum and 1X Penicillin-Streptomycin (Gibco).

Fluorescence:

Article Title: An accessible microfluidic perfusion platform for time-restricted control of zebrafish embryonic patterning
Article Snippet: .. For 4-D fluorescence microscopy, we first fluorescently marked cell nuclei and membranes by injecting 1-cell stage embryos with a mixture of H2B-iRFP, EGFP-CAAX, or mCherry-CAAX mRNAs in vitro transcribed from available plasmids using SP6 polymerase (New England Biolabs, NEB, #E2070), capped with Faustovirus capping enzyme (NEB #M2081) and methylated with mRNA Cap 2′-O-Methyltransferase (NEB #M0366). ..

Microscopy:

In Vitro:

Methylation:

other:

Article Title: Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens.
Article Snippet: For the synthesis of m 1 and mo 5 U-modified FLuc mRNA transcripts, UTP was replaced with either m 1 TP (Trilink, N1081) or mo 5 UTP (Trilink, N-1093) in the reaction mixture, respectively.

Modification:

Article Title: Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens
Article Snippet: Finally, mRNA was purified using Monarch Spin RNA Cleanup Kit (NEB, T2050). .. Cap-1 modified FLuc mRNA was generated using Faustovirus Capping Enzyme (FCE; NEB, M2081S). .. Briefly, 50 μg of FLuc mRNA was diluted to a final volume of 50 μl in FCE capping buffer (50 mM Tris–HCl, 5 mM KCl, 1 mM MgCl 2 , 1 mM DTT, pH 8.0) containing 200 μM S-adenosylmethionine (SAM), 500 μM GTP, 50 units of FCE, and 200 units of mRNA Cap 2′-O-methyltransferase (NEB, M0366).

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